Kinetics, clonality, phenotype, and function of monoclonal and polyclonal CTL in vivo. (A) Percent multimer⁺CD8⁺ T cells (left y axis) in PBMCs (solid circles) collected before and at defined time points after monoclonal (dashed line) and polyclonal (solid line) CTL infusions (indicated). Gray shaded areas indicate anti-CTLA4 treatment. (B and C) Inset pie charts represent individual clonotypes composing the monoclonal (B) and polyclonal (C) infused CTL. Graphs track the corresponding unique (B) and sum of clonotypes (C) as a percentage of total CD8⁺ T cells (y axis). Time points in which the corresponding clones were assessed but not detected (nd) are indicated. *, only clone TCR-13 was detected immediately before the polyclonal infusion with a frequency of 0.054%. (D) Percent expression of CD28, CD62L, CCR7 (long-lived memory markers, blue shade), PD1 (activation/exhaustion marker, red shade), IFN-γ, TNF, and IL-2 (functional markers, green shade) on polyclonal (top) and monoclonal (bottom) infused CTL. (E and F) The same analysis performed on multimer⁺ cells 1 d (E) and 86 d (F) in vivo after infusion.