Altered effector state is G9a methyltransferase dependent. (A) Quantitative RT-PCR analysis of the indicated genes from sorted BM iILC2s expanded for 10 d in IL-2, IL-7, and IL-25 in the presence or absence of UNC0638. Data are from two independent experiments (n = 6–7 per treatment). (B and C) BM iILC2s expanded for 14 d in IL-2, IL-7, and IL-25 and processed for ChIP analysis using antibodies specific for H3K9me2 (B) or H3K9/14Ac (C). Quantitative PCR was performed using primers for the promoters of the specified genes. Data shown are from three to five independent experiments performed in triplicate. *, P < 0.05; Student’s t test. Errors bars indicate SEM.