G9a regulates the development of iILC2s. (A) Frequencies of iILC2s in G9a^fl/fl and Vav.G9a^−/− mice. The numbers show percentages of gated cells (n > 12). (B) Absolute numbers of iILC2s in BM (n = 12 mice per genotype). (C) IL-17RB expression on BM iILC2s from Vav.G9a^−/− mice (black line) and control G9a^fl/fl mice (dark gray; n = 5). The light gray shaded area represents the negative control (Ctrl). (D) ST2 and GATA3 expression in BM iILC2s from G9a^fl/fl or Vav.G9a^−/− mice. Data are representative of two independent experiments (n = 2–3 per experiment). (E) Absolute numbers of BM ILC2s in Vav.G9a^−/− mice and control G9a^fl/fl mice after treatment with PBS or IL-33. Data are from three independent experiments (n = 5 for PBS and n = 11 for IL-33). (F) Reconstitution of mixed BM chimeras of Vav.G9a^−/− BM and control G9a^fl/fl BM competed with CD45.1/2 BM and together injected into lethally irradiated CD45.1 mice was examined 8–12 wk after transplant. (G) Relative iILC2 chimerism normalized to splenic B cells. Data are from two of three independent experiments (n = 4–5 mice per experiment). (H) Frequencies of CLPs (CD45⁺ Lin⁻ CD127⁺ Flt3⁺ α₄β₇⁻), α₄β₇⁺ lymphoid progenitors (αLP; CD45⁺ Lin⁻ CD127⁺ Flt3⁻ α₄β₇⁺), and ChILPs (CD45⁺ Lin⁻ CD127⁺ Flt3⁻ α₄β₇⁺ CD25⁻ c-Kit⁺). Data are from three independent experiments (n = 7). ns, not significant. *, P < 0.05; Student’s t test. Errors bars indicate SEM.