Neutropenia at the time of immunization enhances DC migration and IL-23 production. (A–E) Subsequent IL-17 production enhances LN Cox2 expression that is crucial for late iLN neutrophilia. (A) Analysis of the iLN 24 h after FITC painting. (B) Analysis of intracellular levels of p40 in CD11c⁺ MHC II⁺ DCs 24 h after CFA. (C) LPS-stimulated GM-CSF–derived BMDCs were co-cultured with sorted transgenic 2D2 CD4⁺ T cells and apoptotic neutrophils for 72 h. (D) LysM-DTA mice treated with 5 µg anti-CXCL1 and anti-CXCL2 i.p. every second day after CFA or treated with 40 µg INDO i.p. on days 4, 6, 8, 10, 12, and 14. iLNs were analyzed at day 14 after CFA. A.U., arbitrary units. (E) LysM-DTA mice treated with 100 µg anti–IL-17 antibody i.p. on days 2, 4, and 6. iLNs were analyzed at day 7 after CFA. Data are representative of two independent experiments. n = 5/group. Results are mean ± SEM. *, P < 0.05; **, P < 0.01 (unpaired Student’s t test).