EG is induced in LysM-DTA mice after CFA immunization. (A) BM, spleen, and iLNs 7 d after CFA immunization. (B) Cytometric bead array flow cytometry analysis of serum G-CSF levels in WT and LysM-DTA mice at the indicated time points after CFA immunization. (C) Analysis of CMPs and GMPs in the spleen and BM. Lineage negative (Lin⁻) is defined by CD11b⁻CD11c⁻CD3⁻NK1.1⁻B220⁻F4/80⁻. The images in A are representative of three independent experiments. n = 4–5/group. Data in B and C are representative of two independent experiments. n = 5/group. Results are mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (unpaired Student’s t test).