Specific neutrophil reduction in LysM-DTA mice. (A) No differences were observed in the numbers of splenic CD11c^hi DCs (CD11b⁺ and CD11b⁻), monocyte (Mc) subsets (inflammatory Ly6C⁺ and resident Ly6C⁻), or eosinophils (SSC^hi). (B) The numbers of tissue macrophages such as brain microglia (CD11b⁺F4/80⁺) or peritoneal macrophages (CD11b⁺F4/80^hi) were not altered in LysM-DTA mice. (C) No differences were seen in the numbers of liver (CD68⁺; bar, 50 μm), duodenum (F4/80⁺CD103⁻; bar, 50 μm), or skin (CD68⁺CD11b⁺ and F4/80⁺; bar, 100 μm) macrophages in LysM-DTA mice in comparison to WT mice. Data in A and B are representative of three independent experiments. n = 4–5/group. Results are mean ± SEM. The images in C are representative of three separate mice.