LysM-DTA mice display a marked reduction of neutrophils in the steady state without changes in other myeloid cell populations. (A and B) Flow cytometry analysis of the percentage and absolute numbers of spleen, BM, and blood neutrophils (CD11b⁺Ly6G⁺; A), as well as splenic red pulp macrophages (RPM; F4/80^hi; Mφ) and monocytes (SSC^lowCD11b⁺F4/80⁺; B) in naive WT and LysM-DTA mice. (C) Immunohistochemistry of spleens from naive WT and LysM-DTA mice to visualize neutrophils (CD11b⁺Ly6G⁺; top), red pulp macrophages (F4/80⁺; middle), and MARCO⁺ and MOMA-1⁺ macrophages (bottom). Representative data are from a minimum of three independent experiments. n = 4–6/group. Results are mean ± SEM. Immunohistochemistry was performed on four separate mice. Bar,100 µm. *, P < 0.05; **, P < 0.01 (unpaired Student’s t test).