Figure 5. cIAP1 and cIAP2 expression is induced by TNF in vitro and is NF-κB dependent in vivo. (A) TCRhi CD8SP thymocytes from F5 Rag1−/− donors were sort purified and stimulated with TNF (n = 4) or PBS (n = 4) as control. mRNA was isolated from cultured and uncultured cells (0 h, n = 3) and gene expression determined by RNAseq analysis. Bar charts show mRNA expression level (normalized reads per kb exons per million reads, nRPKM) of the indicated genes. Names in brackets indicate protein name associated with gene. (B) TCRhi CD8SP thymocytes were sorted from WT (n = 3) and Tnfrsf1a−/− IKKΔTCD4 mice (n = 4). mRNA was purified and gene expression was determined by RNAseq analysis. Bar charts show mRNA expression level (nRPKM) of the indicated genes. Individual RNAseq libraries were generated from independent cell preparation and treatments. *, P < 0.05.
Figure 5.

cIAP1 and cIAP2 expression is induced by TNF in vitro and is NF-κB dependent in vivo. (A) TCRhi CD8SP thymocytes from F5 Rag1−/− donors were sort purified and stimulated with TNF (n = 4) or PBS (n = 4) as control. mRNA was isolated from cultured and uncultured cells (0 h, n = 3) and gene expression determined by RNAseq analysis. Bar charts show mRNA expression level (normalized reads per kb exons per million reads, nRPKM) of the indicated genes. Names in brackets indicate protein name associated with gene. (B) TCRhi CD8SP thymocytes were sorted from WT (n = 3) and Tnfrsf1a−/− IKKΔTCD4 mice (n = 4). mRNA was purified and gene expression was determined by RNAseq analysis. Bar charts show mRNA expression level (nRPKM) of the indicated genes. Individual RNAseq libraries were generated from independent cell preparation and treatments. *, P < 0.05.

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