TNF blockade rescues the developmental block in pLck IκB-PEST mice. pLck IκB-PEST mice were treated with anti-TNF (n = 8; 1 mg, days 0, 2, and 4) or PBS (n = 8) injection as control. At day 7, thymi were analyzed by FACS. Density plots are of CD4 versus CD8 by total live (top row) and HSA versus TCR by CD8SP gated thymocytes from pLck IκB-PEST mice treated with either anti-TNF (αTNF) or PBS as control. Transgene -ve (WT, n = 4) littermates were also analyzed as control. Bar charts show total cell numbers of the indicated thymocyte subset recovered from the indicated treatment groups. Data are pooled from two independent experiments. **, P < 0.01.