RNAseq analysis of CMPs. (A) FL and adult BM CMPs (n = 3 biological replicates for each condition isolated over at least two separate sorting experiments) were compared by RNAseq to identify significantly different genes between the two populations at a false discovery rate (FDR) cutoff of <0.001. GSEA was performed to compare adult BM CMP- and FL CMP-specific transcripts to normal adult promyelocyte, myelocyte, granulocyte, and erythroblast transcriptomes. In the top row, a significant positive correlation was observed between adult BM CMP-specific transcripts and promyelocyte-enriched transcripts (left), indicating enrichment of adult BM CMP-specific transcripts among transcripts increased in promyelocytes relative to erythroblasts. A significant negative correlation was observed between FL CMP-specific transcripts (right) and transcripts increased in promyelocytes relative to erythroblasts, indicating correlation with erythroblast-specific transcripts. Normalized enrichment score (NES), nominal p-value (NOM p), and FDR are presented. The same analysis was performed in the middle with myelocyte transcripts and at the bottom with granulocyte transcripts. (B) Heat maps showing expression of erythroid/platelet-specific and granulocyte/monocyte-specific transcripts measured by RNAseq are presented.