Similar PrP^Sc deposition, microglial activation, and astrogliosis in terminally sick IL34^−/− and IL34^+/+ mice. (A) H&E and SAF84 immunohistochemistry of the hippocampi of terminally sick RML6-infected IL34^−/− and IL34^+/+ (WT) mice. Bars, 50 µm. (B, top) PrP^Sc Western blot of hemispheric brain homogenates prepared from terminally sick RML6-infected IL34^−/− and IL34^+/+ (WT) mice. (bottom) Densitometric quantification of the PrP^Sc Western blot. n = 5. (C) Iba1 immunohistochemistry of hippocampi and cortex from terminally sick RML6-infected IL34^−/− and IL34^+/+ (WT) mice. Bars, 20 µm. (D, top) Iba1 Western blot of hemispheric brain homogenates prepared from terminally sick RML6-infected IL34^−/− and IL34^+/+ (WT) mice. (bottom) Densitometric quantification of the Iba1 Western blot. n = 5. (E) GFAP immunohistochemistry of hippocampi and cortex from terminally sick RML6-infected IL34^−/− and IL34^+/+ (WT) mice. Bars, 20 µm. (F, top) GFAP Western blot of hemispheric brain homogenates prepared from terminally sick RML6-infected IL34^−/− and IL34^+/+ (WT) mice. (bottom) Densitometric quantification of the GFAP Western blot. n = 5. Error bars represent SEM. Statistical significance in B, D, and F was determined using unpaired Student’s t test. Histology and Western blot results represent at least five independent experiments.