Dpy30 KO results in loss of long-term maintenance of HSCs. (A and B) Donor contribution in BM chimeras 3 (A) and 5 (B) mo after pIpC injections following the scheme in Fig. 4 A. n = 10 mice of each genotype for PB; n = 4 or 5 mice of each genotype for BM and thymus. P < 0.001 for all cell populations by Student’s t test. (C) Donor contribution in recipients 3 mo after transplantation of LT-HSCs purified from pIpC-injected Mx1-Cre; Dpy30^F/+ or Mx1-Cre; Dpy30^F/– mice. n = 6 mice for each genotype. P < 0.01 for all cell populations by Student’s t test. (D) Donor contribution 3 mo after secondary transplantation, in which donor-derived HSCs sorted from pIpC-injected BM chimeras (primary recipients) were competitively transplanted into secondary recipient mice. n = 4 or 5 mice for each genotype. P < 0.05 for all cell populations by Student’s t test, except for CD4⁺ and CD8⁺ thymocytes, for which the levels in control genotype were already extremely low and highly variable, preventing a manifestation of a statistically significant reduction in the KO background. Data are shown as mean ± SD for all panels.