Impaired induction of lineage-regulatory genes after loss of Dpy30. (A–C) Gene expression analyses were performed for Mx1-Cre; Dpy30^F/+ or Mx1-Cre; Dpy30^F/– donor-derived cells 2 wk after pIpC injections after the schemes in Fig. 4 (A and C). (A) RNA amount per cell in sorted cell populations. n = 3 mice for each genotype. P > 0.1 for all by Student’s t test. Data are shown as mean ± SD. (B) Expression change (shown as Log₂ fold change) from LSK to MyePro cells in F/− (red) and in F/+ (blue) backgrounds, as determined by RNA-seq of sorted cells. Genes (represented by each dot) were ranked according to their fold changes in F/+ cells, and only genes with fold change >2 (Log₂ fold change >1) in F/+ cells are shown. Note that most of these genes show a smaller fold change in F/− than in F/+ cells. See Table S2 for the gene lists. (C) Expression levels of indicated genes in the sorted cell populations were determined by RT-qPCR and normalized to Actb, and shown as mean ± SD of duplicate measurements. The expression levels in F/+ LSK cells were set as 1. Shown are representative results from one out of three independent chimera transplantation experiments. Results from two more independent transplantation experiments (not depicted) are consistent with these results.