Figure 4.
Figure 4. Loss of NFκB1 in B cells is linked with deregulated IL-6 production. (A) Relative mRNA expression of 84 genes associated with inflammation and autoimmunity was analyzed in Fo B cells from the LNs of 6–8-wk-old WT and Nfκb1−/− mice. Genes in red denote statistical significance (P < 0.05). Data are derived from three independent experiments, each with pooled samples from four mice per genotype (n = 12). (B) Serum cytokine levels in BM-reconstituted mice at the indicated time points (each symbol represents one mouse; two or three independent experiments). P-values as indicated were determined by unpaired Student's t test. (C) Spleen cryosections were stained with anti-B220 (B cells; red) or PNA lectin (GG; red) combined with anti–IL-6 (green). Colocalization is shown in yellow (n = 4 mice/group; three independent experiments). Bar, 50 µm.

Loss of NFκB1 in B cells is linked with deregulated IL-6 production. (A) Relative mRNA expression of 84 genes associated with inflammation and autoimmunity was analyzed in Fo B cells from the LNs of 6–8-wk-old WT and Nfκb1−/− mice. Genes in red denote statistical significance (P < 0.05). Data are derived from three independent experiments, each with pooled samples from four mice per genotype (n = 12). (B) Serum cytokine levels in BM-reconstituted mice at the indicated time points (each symbol represents one mouse; two or three independent experiments). P-values as indicated were determined by unpaired Student's t test. (C) Spleen cryosections were stained with anti-B220 (B cells; red) or PNA lectin (GG; red) combined with anti–IL-6 (green). Colocalization is shown in yellow (n = 4 mice/group; three independent experiments). Bar, 50 µm.

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