Recognition of 16.113p escape variants by TCR-IV T cells is IFN-γ-dependent as well as the rejection of Tet-TagLuc tumors. (A) Levels of secreted IFN-γ by TCR-I– or TCR-IV–transduced CD8⁺ OT-1/Rag^−/− T cells upon 24-h co-culture with 16.113p and 999 cells (with [+] or without [−] 100 ng/48 h IFN-γ pretreatment) measured by an IFN-γ ELISA assay (E:T/2:1). Combined data from two experiments are shown, and error bars indicate SD. The remaining escape variants from the same experiments are shown in Fig. S2 C. (B) Tumor volumes over time from Rag^−/− mice bearing Tet-TagLuc tumors treated with TCR-I, TCR-IV, or mock T cells derived from OT-1/IFN-γ^−/−/Rag^−/− mice. Each line reflects an individual mouse and the bottom table shows total mice numbers from two experiments.