Similar avidity for TCR-I and TCR-IV T cells. (A) Untransduced and TCR-I– or TCR-IV–transduced CD8+ P14/Rag−/− T cells labeled with pI- or pIV-specific tetramers and gated on total lymphocytes. One representative of four experiments is shown. (B) The time needed for dissociation of 50% of bound pMHC complexes (T1/2) from the surface of an individual T cell is indicated in seconds (TCR-I, n = 21; TCR-IV, n = 12). Combined data from two experiments. The two datasets were compared using a two-sided Wilcoxon rank sum test (P = 0.002). (C) IFN-γ values obtained by co-culturing TCR-I and TCR-IV T cells with equal numbers of RMA-S cells pulsed with different concentrations of pI or pIV. The values are given as percentage of maximum, which was calculated by taking the T cell response to the highest peptide concentration as 100% (maximal value), and then calculating the percentage of for each value as percentage of maximum. One representative experiment of two is shown.
