Characterization of human CD112R protein. (A) Protein sequence encoded by the human CD112R gene. Predicted extracellular IgV-like and transmembrane domains are highlighted in blue and red, respectively. Two tyrosines (Y233 and Y293) in the cytoplasmic domain are underlined with one within an ITIM-like motif underlined. (B) Alignment of the extracellular domains of human and mouse CD112R protein sequences using the MacVector 6.5 program. The shaded boxes refer to the shared amino acids among CD112R orthologues. (C) Guide tree analysis of human CD112R and the known PVR-like proteins via the Clustal W program in MacVector 6.5. (D) Multiple sequence alignment of the IgV domains of PVR-like proteins. Similar and identical residues among this group are shaded in red. The PVR signature motifs are outlined in green frames. Blue boxes mark conserved amino acids. (E) A predicted protein structure model of human CD112R IgV domain (55–150 aa) using human PVRL4 (Protein Data Bank accession no. 4JJH) as the template.