Figure 3. Dedifferentiation of aortic SMCs in elastin nulls. (A) Transverse aortic sections of wild-type and Eln(−/−) pups at P0.5 were stained for SMA, SMMHC, CD31, and nuclei (DAPI). (B) SMMHC mRNA levels normalized to Gapdh as measured by RT-PCR from aortas of specified genotype at P0.5; n = 3 aortas in duplicate. (C) Aortic sections of pups as in A were stained for SMA, PDGFR-β, and DAPI. At P0.5, PDGFR-β marks outer layer SMA− cells (i.e., adventitial cells). Note the hyperplasia of both smooth muscle and adventitia of the elastin-null aorta. (D) Cells (DAPI+ nuclei) of descending aortic wall of the specified elastin genotypes were scored as SMCs (PDGFR-β−SMA+) or adventitial cells (PDGFR-β+SMA−). The total numbers of scored cells were 528, 584, and 778 for wild type, Eln(+/−), and Eln(−/−), respectively. For each genotype, n = 3 pups. Data are presented as mean ± SD. ANOVA was used. *, P < 0.05. Lu, aortic lumen; M, media; A, adventitia. Bars, 10 µm.
Figure 3.

Dedifferentiation of aortic SMCs in elastin nulls. (A) Transverse aortic sections of wild-type and Eln(−/−) pups at P0.5 were stained for SMA, SMMHC, CD31, and nuclei (DAPI). (B) SMMHC mRNA levels normalized to Gapdh as measured by RT-PCR from aortas of specified genotype at P0.5; n = 3 aortas in duplicate. (C) Aortic sections of pups as in A were stained for SMA, PDGFR-β, and DAPI. At P0.5, PDGFR-β marks outer layer SMA− cells (i.e., adventitial cells). Note the hyperplasia of both smooth muscle and adventitia of the elastin-null aorta. (D) Cells (DAPI+ nuclei) of descending aortic wall of the specified elastin genotypes were scored as SMCs (PDGFR-β−SMA+) or adventitial cells (PDGFR-β+SMA−). The total numbers of scored cells were 528, 584, and 778 for wild type, Eln(+/−), and Eln(−/−), respectively. For each genotype, n = 3 pups. Data are presented as mean ± SD. ANOVA was used. *, P < 0.05. Lu, aortic lumen; M, media; A, adventitia. Bars, 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal