Figure 5. PTPN11 D61Y cooperates with AML1-ETO to induce leukemia in vivo. (A) Kaplan-Meier plot of mouse survival after transplantation with cells transduced with the given vector. WT pBEX (n = 10); WT AE (n = 15); PTPN11 D61Y pBEX (n = 10); PTPN11 D61Y AE (n = 15). (B) Peripheral blood counts at euthanasia of leukemic PTPN11 D61Y AE mice (n = 5), as well as controls (from left to right, n = 10, n = 15, and n = 10). Horizontal bars represent mean. P-values were generated using the Student’s t test: *, P < 0.05; ****, P < 0.0001; n.s., not significant. (C) Representative hematoxylin and eosin staining (n = 3). Bars, 50 µm. (D) At euthanasia, leukemic mice exhibit splenomegaly. From top to bottom: liver, kidney, and spleen. (E) Representative flow cytometry analysis of the bone marrow of transplant recipients (n = 3).
Figure 5.

PTPN11 D61Y cooperates with AML1-ETO to induce leukemia in vivo. (A) Kaplan-Meier plot of mouse survival after transplantation with cells transduced with the given vector. WT pBEX (n = 10); WT AE (n = 15); PTPN11 D61Y pBEX (n = 10); PTPN11 D61Y AE (n = 15). (B) Peripheral blood counts at euthanasia of leukemic PTPN11 D61Y AE mice (n = 5), as well as controls (from left to right, n = 10, n = 15, and n = 10). Horizontal bars represent mean. P-values were generated using the Student’s t test: *, P < 0.05; ****, P < 0.0001; n.s., not significant. (C) Representative hematoxylin and eosin staining (n = 3). Bars, 50 µm. (D) At euthanasia, leukemic mice exhibit splenomegaly. From top to bottom: liver, kidney, and spleen. (E) Representative flow cytometry analysis of the bone marrow of transplant recipients (n = 3).

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