MyD88 deficiency has different effect on diabetes development. (A–E) Individual TLR- or MyD88^−/−NY8.3NOD mice were generated by breeding different TLR^−/− or MyD88^−/−NOD mice with NY8.3 NOD mice. Diabetes development was observed, and data were pooled from at least five independent experiments. (A) TLR2^−/−NY8.3. (B) TLR4^−/−NY8.3. (C) TLR5^−/−NY8.3. (D) TLR9^−/−NY8.3. (E) MyD88^−/−NY8.3. Wilcoxon test for survival was used for analysis of diabetes incidence. *, P < 0.05; **, P < 0.01; ***, P < 0.001. F, female. M, male.