M-CSF treatment does not affect platelet recovery or blood glucose levels. (a–c) Mice were transplanted with actin-GFP HS/PCs and treated with rmM-CSF, rhG-CSF, or PBS as described in Fig. 1 a and analyzed for donor side scatter (SSC)^lo forward scatter (FSC)^lo platelet contribution in the blood shown as time course of median absolute numbers (b) or quantification at indicated time points (c). Non-irradiated (noXR) nontransplanted controls were used as a reference. For 1 wk, * = 0.011 (>0.01 and <0.06). For 7 wk, * = 0.06. (d) Time course of weight and blood glucose levels of mice transplanted and treated with rmM-CSF (n = 6) or PBS (n = 6) as indicated in Fig. 1 a. Error bars represent the median and error with interquartile range. **, P < 0.01; ***, P < 0.001 by Mann-Whitney U tests. Data are representative of two independent experiments.