GATA1 target Bcl-xL plays a role in CMS polycythemia and erythroid progenitors. (A) Western blot analysis confirming the overexpression of Bcl-xL in the non-CMS levels. Lane 1 represents the non-CMS cell line infected with scrambled control. Lane 2 represents non-CMS cells overexpressing Bcl-xL construct. Lane 3 shows the expression levels on uninfected non-CMS cells. Each bar represents a mean of three replicates, and the experiment was repeated at least twice. Error bars represent SEM. **, P < 0.01. (B) Overexpression (OE) of Bcl-xL partially rescues the blunted response of non-CMS cells (gray bar). Error bars represent mean ± SEM. Measurements were done in triplicates. The experiment was repeated three times. The response of scrambled control (pink bar) as well as sea-level (green), CMS (red), and non-CMS (blue) uninfected cells are also shown. *, P < 0.05; Student’s t test. Scramb, scrambled. (C) Overexpression of Bcl-xL increases the number of BFU-e in non-CMS cells (gray). SENP1KO decreases the number of colonies in CMS cells (brown). *, P < 0.05; Student’s t test. Each bar represents the mean, and error bars represent SE. For each sample, the levels were measured in triplicates. Each experiment was repeated three times.