Pro–B cells from HDAC7-deficient mice express genes from alternative lineages. (A) KEGG pathway enrichment and Gene Ontology (GO) analysis of up-regulated genes in HDAC7-deficient pro–B cells. (B) Representative FACS analyses from three independent experiments showing Mac-1 expression in gated bone marrow lymphocytes from Hdac7^+/− and Hdac7^fl/− mice (top). Histogram showing Mac-1 expression in gated bone marrow lymphocytes from Hdac7^+/− (red) and Hdac7^fl/− (blue) mice (bottom). FSC, forward scatter. (C) Representative FACS analyses from three independent experiments showing Mac-1 expression in purified CD19⁺ bone marrow lymphocytes from Hdac7^+/− and Hdac7^fl/− mice. (D) Heatmap showing the expression pattern of HDAC7 and selected myeloid genes in different hematopoietic cell subsets. Data were obtained from the Immunological Genome Project. A description of the different cell subsets analyzed can be found at www.Immgen.org. (E) RT–quantitative PCR experiments for gene expression changes for selected up-regulated genes in the absence of HDAC7. *, P < 0.05; **, P < 0.01. (F) RT–quantitative PCR experiments for gene expression levels of B cell genes. (E and F) Data are represented as the mean ± SEM of three independent experiments. (G) Western blot analysis for the expression of HDAC7 and B cell transcription factors in wild-type and HDAC7-deficient B lymphocytes.