Protein expression analysis in NFKBIE-deleted versus WT CLL. (A) Protein expression profiles of IκBα, IκBβ and IκBε, p65, and phospho-p65 in NFKBIE-deleted (n = 4) versus WT (n = 4) CLL by Western blot analysis. (B) Protein expression profiles of IκBα, IκBβ and IκBε, p65, and phospho-p65 in additional NFKBIE-deleted (n = 3) versus WT (n = 3) CLL by Western blot analysis. (C) Mean normalized protein expression values for IκBα, IκBβ and IκBε, p65, and phospho-p65 in NFKBIE-deleted (n = 7) versus WT CLL (n = 7). * indicates P < 0.05, whereas *** indicates P < 0.001. For IκBβ, only four NFKBIE-deleted and four WT CLL cases were assessed. Error bars indicate standard error. (D) Western blot analysis of IκBε in CLL to identify the presence of a truncated IκBε protein. The membrane is overexposed. del, deleted.