ASCIZ regulates B cell development via DYNLL1. (A) Western blot analysis of DYNLL1 levels in FACS-isolated bone marrow B220⁺IgM⁻ cells of two controls and two Mb1/Mb1-Cre Asciz^fl/fl deleters. GAPDH is used as loading control. Molecular mass standards are indicated on the right. (B) Reconstitution of spleens of lethally irradiated recipients with Mb1/Mb1-Cre Asciz^fl/fl deleter–derived cells that were retrovirally transduced with MigR1 empty vector (control; n = 2) or MigR1-Dynll1 (n = 3). The graph shows the percentage of B220⁺IgM⁺ spleen cells among transduced GFP-positive donor-derived cells. FACS plots (below) indicate the gating strategy. Ly5.2-positive donor-derived cells were gated for IRES-GFP expression to detect vector (left)- or Dynll1 (right)-transduced cells. Error bars represent mean ± SEM. Each symbol represents one animal. All data were obtained in one experiment.