Figure 4. Mb1-Cre Asciz deletion leads to progressive B cell loss during B cell development. (A) Photograph of representative spleens from Mb1-Cre−Ascizfl/fl, Mb1/Mb1-Cre Asciz+/+ controls, and an Mb1/Mb1-Cre Ascizfl/fl deleter (left) and relative spleen weights of controls (n = 28) and deleters (n = 36; right). (B) Representative FACS plots of spleens isolated from an ASCIZ-Mb control and a deleter (left) and absolute numbers of splenic B220+IgM+ B cells in controls (n = 20) and Mb1/Mb1-Cre Ascizfl/fl deleters (n = 28; right). The control group contains both Mb1-Cre−Ascizfl/fl and Mb1/Mb1-Cre Asciz+/+ animals. (C) Representative FACS plots of bone marrows from an ASCIZ-Mb control and an Mb1/Mb1-Cre Ascizfl/fl deleter (left) and absolute cell numbers (right) of B220lowIgM+ immature B cells (gated as indicated on the FACS plots) in a single hind leg of controls (n = 9) and deleters (n = 14). The control group contains both Mb1-Cre−Ascizfl/fl and Mb1/Mb1-Cre Asciz+/+ animals. (D) Representative FACS plots of B220+IgM−-gated bone marrow cells from an ASCIZ-Mb control and an Mb1/Mb1-Cre Ascizfl/fl deleter stained for CD43 and CD19 (left). The right panel shows grouped data for pro-B cells (B220+IgM−CD43+CD19+) and pre-B cells (B220+IgM−CD43−CD19+) in controls (n = 9) and deleters (n = 9). The control group contains both Mb1-Cre−Ascizfl/fl and Mb1/Mb1-Cre Asciz+/+ animals. (E) Representative FACS plots of cell death (AnnexinV+7-AAD+) in immature and transitional B cells (B220+IgM+IgD−/low) in an ASCIZ-Mb control and a deleter (left) and grouped data for Mb1/Mb1-Cre Asciz+/+ controls (n = 5) and Mb1/Mb1-Cre Ascizfl/fl deleters (n = 4). (A–E) Each data point represents one mouse. All grouped data represent mean ± SEM. Data were obtained in at least two independent experiments. Closed circles indicate controls; open circles indicate deleters.
Figure 4.

Mb1-Cre Asciz deletion leads to progressive B cell loss during B cell development. (A) Photograph of representative spleens from Mb1-Cre−Ascizfl/fl, Mb1/Mb1-Cre Asciz+/+ controls, and an Mb1/Mb1-Cre Ascizfl/fl deleter (left) and relative spleen weights of controls (n = 28) and deleters (n = 36; right). (B) Representative FACS plots of spleens isolated from an ASCIZ-Mb control and a deleter (left) and absolute numbers of splenic B220+IgM+ B cells in controls (n = 20) and Mb1/Mb1-Cre Ascizfl/fl deleters (n = 28; right). The control group contains both Mb1-Cre−Ascizfl/fl and Mb1/Mb1-Cre Asciz+/+ animals. (C) Representative FACS plots of bone marrows from an ASCIZ-Mb control and an Mb1/Mb1-Cre Ascizfl/fl deleter (left) and absolute cell numbers (right) of B220lowIgM+ immature B cells (gated as indicated on the FACS plots) in a single hind leg of controls (n = 9) and deleters (n = 14). The control group contains both Mb1-Cre−Ascizfl/fl and Mb1/Mb1-Cre Asciz+/+ animals. (D) Representative FACS plots of B220+IgM−-gated bone marrow cells from an ASCIZ-Mb control and an Mb1/Mb1-Cre Ascizfl/fl deleter stained for CD43 and CD19 (left). The right panel shows grouped data for pro-B cells (B220+IgM−CD43+CD19+) and pre-B cells (B220+IgM−CD43−CD19+) in controls (n = 9) and deleters (n = 9). The control group contains both Mb1-Cre−Ascizfl/fl and Mb1/Mb1-Cre Asciz+/+ animals. (E) Representative FACS plots of cell death (AnnexinV+7-AAD+) in immature and transitional B cells (B220+IgM+IgD−/low) in an ASCIZ-Mb control and a deleter (left) and grouped data for Mb1/Mb1-Cre Asciz+/+ controls (n = 5) and Mb1/Mb1-Cre Ascizfl/fl deleters (n = 4). (A–E) Each data point represents one mouse. All grouped data represent mean ± SEM. Data were obtained in at least two independent experiments. Closed circles indicate controls; open circles indicate deleters.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal