Normal T cell development in Mx1-Cre Asciz deleters. (A) Western blot of thymic protein levels of two different ASCIZ-Mx deleters and two controls probed for ASCIZ and actin as a loading control at 4 wk after poly(I:C) injection. Molecular mass standards are indicated on the right. (B) Representative FACS plots of blood isolated from ASCIZ-Mx controls and deleters (left) stained for CD4 and CD8 and absolute blood T cell numbers at 4 wk after poly(I:C) injection (right). n = 11 for controls (n = 5 Mx1-Cre⁺Asciz^fl/+ and n = 6 Mx1-Cre⁻Asciz^fl/fl), and n = 8 for Mx1-Cre⁺Asciz^fl/fl deleters. (C) Representative FACS plots of a thymus isolated from an ASCIZ-Mx control and a deleter thymus (left) and absolute thymic T cell numbers (right) at 4 wk after poly(I:C) injection. n = 15 for controls (n = 9 Mx1-Cre⁺Asciz^fl/+ and n = 6 Mx1-Cre⁻Asciz^fl/fl), and n = 8 for Mx1-Cre⁺Asciz^fl/fl deleters. (B and C) All grouped data represent mean ± SEM. Similar results were obtained at 16 wk after poly(I:C) injection.