Figure 5. Caspase-1 and ASC activation in Wdr1 mutant monocytes. E14.5 fetal livers from Wdr1+/rd or Wdr1xn/rd embryos were cultured in L929 cell-conditioned medium for 3 d. (a and b) 105 FLDMs from day 3 cultures were incubated with 1 µg/ml LPS (a) or treated with LPS ± VX-765 (b). 48 h after stimulation, culture supernatants were harvested and assayed by ELISA for IL-18. (c) 24 h after stimulation, cells were fixed and permeabilized, with intracellular ASC specks stained and then imaged by confocal microscopy. All data are representative of two to four independent experiments; error bars represent means ± SEM (n = 3–6). *, P < 0.05; ***, P < 0.001; ****, P < 0.0001 by unpaired t test.
Figure 5.

Caspase-1 and ASC activation in Wdr1 mutant monocytes. E14.5 fetal livers from Wdr1+/rd or Wdr1xn/rd embryos were cultured in L929 cell-conditioned medium for 3 d. (a and b) 105 FLDMs from day 3 cultures were incubated with 1 µg/ml LPS (a) or treated with LPS ± VX-765 (b). 48 h after stimulation, culture supernatants were harvested and assayed by ELISA for IL-18. (c) 24 h after stimulation, cells were fixed and permeabilized, with intracellular ASC specks stained and then imaged by confocal microscopy. All data are representative of two to four independent experiments; error bars represent means ± SEM (n = 3–6). *, P < 0.05; ***, P < 0.001; ****, P < 0.0001 by unpaired t test.

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