Clodronate liposome depletion of circulating monocytes prevents Wdr1^rd/rd autoinflammatory disease. Lethally irradiated chimeric mice containing Wdr1^rd/rd BM were injected intravenously with clodronate liposomes (Clo-lipo) or PBS liposomes (PBS-lipo) twice per week starting from 2 wk after BM reconstitution. (a) Incidence of inflammation from chimeric mice after BM reconstitution. (b) After eight injections of liposomes, two representative mice from each group were photographed, and an ear from each mouse is highlighted. (c and d) 24 h after the fourth injection of liposomes, peripheral blood leukocytes were analyzed by flow cytometry as indicated by pink boxes (c) and quantified as CD115⁺Gr1⁻ resident monocytes, CD115⁺Gr1⁺ inflammatory monocytes, and CD115⁻Gr1^hi neutrophils (d). Error bars represent means ± SEM (n = 5). **, P < 0.01 by Gehan-Breslow-Wilcoxon test; ***, P < 0.001 by unpaired t test. All data are representative of two independent experiments.