12/15-lipoxygenase does not restore viability of Gpx4 deficient T cells. (A) Splenic CD4⁺ and CD8⁺ T cell population (left) and numbers (right) of WT, T^{ΔGpx4/ΔGpx4}, 12/15-lipoxygenase (Alox15^−/−), and T^{ΔGpx4/ΔGpx4} crossed with 12/15-lipoxygenase (T^{ΔGpx4/ΔGpx4/}Alox15^−/−; n = 3 per group of 6 wk old mice). Flow cytometry (left) and total number (right) of gp_33-41 tetramer specific CD8⁺ cells from LCMV-infected spleens of WT, T^{ΔGpx4/ΔGpx4}, Alox15^−/−, and T^{ΔGpx4/ΔGpx4/}Alox15^−/− (B; n ≥ 4 per group). WT, T^{ΔGpx4/ΔGpx4}, Ripk3^−/−, and T^{ΔGpx4/ΔGpx4/}Ripk3^−/− (C; n = 3 per group) at 7 dpi. (D and E) Necrostatin-1 (Nec-1; 1.65 mg/kg) was injected daily beginning from 2 d before infection with LCMV 200 pfu WE strain. Total number of splenic CD4⁺ and CD8⁺ T cells (D) and gp33-41 tetramer specific CD8⁺ T cells (E) at 7 dpi (n = 3 per group). Representative data are shown from two independent experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (two-tailed Student’s t test).