Developmental pathways are modulated by WSS. (A) Gene expression was determined by Illumina WG-6 BeadChips in E10.5 AGM-derived cells after 6 or 36 h of culture with WSS or static conditions. The Venn diagram displays overlap of genes differentially expressed between static and WSS at 6 and 36 h (n = 3 independent experiments at each time point; unpaired Student’s t test: P < 0.01; twofold threshold). Genes listed in red are up-regulated with application of WSS, whereas genes in blue are down-regulated. (B) Ingenuity pathway analysis reveals roles for WSS in several canonical pathways. (C) Functional themes are identified by network visualization of gene sets enriched by WSS at 6 and 36 h (p-value cutoff 0.05, FDR 0.8, overlap coefficient cutoff 0.5). Each node represents between 10 and 600 genes, where distance between nodes and thickness of edge lines reflects overlap of genes. Direction of enrichment is depicted for 6 h as color of inner node and for 36 h as color of node border (up, red; down, blue).