Figure 4. Hemogenic precursors of Ncx1 mutant embryos lack instructive signaling from Runx1, Notch, and Wnt. (A) E9.5 PSps were isolated from individual embryos and profiled for cell surface markers after dissociation. (B) Quantification of populations within Ncx1 mutant embryos showed elevated percentages of hemogenic endothelial and hematopoietic cells, reflecting an ability to specify blood lineages but an impairment in the cells ability to leave the PSp because of lack of circulation. Frequency of hemogenic populations is elevated in Ncx1−/− mutants (n = 5 wild-type littermates, n = 3 Ncx1−/−; unpaired Student’s t test: *, P = 0.015; **, P < 0.006). (C) Ncx1−/− embryos express aberrantly low levels of critical regulators of hematopoiesis. Transcripts of key developmental pathways are reduced in Ncx1−/− PSp (n = 4–6 wild-type littermates, n = 5–6 Ncx1−/−; two-tailed Student’s t test: *, P < 0.03; **, P < 0.009). Error bars represent SEM.
Figure 4.

Hemogenic precursors of Ncx1 mutant embryos lack instructive signaling from Runx1, Notch, and Wnt. (A) E9.5 PSps were isolated from individual embryos and profiled for cell surface markers after dissociation. (B) Quantification of populations within Ncx1 mutant embryos showed elevated percentages of hemogenic endothelial and hematopoietic cells, reflecting an ability to specify blood lineages but an impairment in the cells ability to leave the PSp because of lack of circulation. Frequency of hemogenic populations is elevated in Ncx1−/− mutants (n = 5 wild-type littermates, n = 3 Ncx1−/−; unpaired Student’s t test: *, P = 0.015; **, P < 0.006). (C) Ncx1−/− embryos express aberrantly low levels of critical regulators of hematopoiesis. Transcripts of key developmental pathways are reduced in Ncx1−/− PSp (n = 4–6 wild-type littermates, n = 5–6 Ncx1−/−; two-tailed Student’s t test: *, P < 0.03; **, P < 0.009). Error bars represent SEM.

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