hpre-CDCs descend from hCDPs. (a) Flow cytometry plots show comparison of hCDP (purple) and hpre-CDC (orange) in cord blood. (b) Flow cytometry plots of gated CD45⁺Lin(CD3/19/56/14)⁻CD34⁺ show phenotype and purity of magnetically enriched cord blood (CB) CD34⁺ cells (presorting, upper) and sorted cells (post-sorting, bottom). hCDPs were gated as CD34⁺CD38^hiCD45RA⁺CD10⁻CD123^hi. (c) Differentiation kinetics of hCDPs purified from CB in MS5+FSG cultures for 1, 2, or 4 d. FACs plots show culture output of live human CD45⁺ cells, including CD34⁺ cells (purple), pDC (green), CD1c⁺ cDC (blue), CD141⁺ cDCs (red), and hpre-CDCs (orange). Representative of four independent experiments are shown. Graphs summarize composition of indicated populations among total hCD45⁺ cells. Bars are mean values from four independent experiments, and error bars are SEM.