Notch blockade in the bone marrow by γ-secretase inhibitor impaired thymic T cell development, whereas Notch overexpression rescued T lymphoid defect. (A) 2.5 mM DAPT or PBS was injected into the left femur of each of 8 C57BL/6J mice at day −12, −9, −6. At day 0, the left femur, thymus, and peripheral blood were harvested for enumeration of bone marrow CLPs, thymic T cell progenitors, and peripheral blood mature T cells by flow cytometry. Experiment was performed twice independently; n = 4–8 mice/group. Data show mean ± SEM. (B) 106 Lineage-depleted hematopoietic progenitors were flow sorted from poly(I:C)-induced Mx1Cre;LSL-NICD-GFP mice or Mx1Cre;LSL-GFP control mice and transplanted into either OcnCre;iDTR mutant or control recipients. Recipients were harvested 1 wk after transplant and bone marrow CLPs, thymic T cell intermediates, and mature peripheral blood T cells were analyzed by flow cytometry. Experiment was performed twice independently; n = 8 mice/group. Data show mean ± SEM.
