Conditional ablation of Notch signaling in hematopoietic cells impaired T lymphopoiesis. (A) Mx1Cre^+/−;Pofut1^Fl/Fl mutants and Mx1Cre^+/−;Pofut^+/+ control littermates were injected with 12.5 µg/g BW polyinosinic:polycytidylic acid (poly(I:C)) every alternate day for 7 d to induce deletion of the Pofut1 gene. Mice were harvested 4 wk after injection and bone marrow CLPs, thymic T cell progenitors, and peripheral blood mature T cells were enumerated by flow cytometry. Experiment was performed twice independently; n = 4–6 mice/group. Data show mean ± SEM. (B) Mx1Cre^+/−;RBPjk^Fl/Fl mutants and Mx1Cre^+/−;RBPjk^+/+ control littermates were injected with 12.5 µg/g BW poly(I:C) every alternate day for 7 d to induce deletion of RBP-Jκ. Mice were harvested 4 wk after injection and bone marrow CLPs, thymic T cell progenitors, and peripheral blood mature T cells were enumerated by flow cytometry. Experiment was performed twice independently; n = 6–8 mice/group. Data show mean ± SEM.