Ocn+ cell regulation of T lymphopoiesis is dependent on bone marrow microenvironment. (A) 106 WT CD45.1 hematopoietic bone marrow cells were transplanted into lethally irradiated CD45.1 OcnCre;iDTR hosts that were untreated or treated with DT. Animals were bled at 8, 12, and 16 wk after transplantation for blood count and assessment of hematopoietic reconstitution by flow cytometry. (B) 5 × 105 bone marrow cells from either control or mutant CD45.2 OcnCre+/−;iDTR donors were mixed with 5 × 105 CD45.1 SJL bone marrow competitors in a 1:1 ratio and transplanted into lethally irradiated WT SJL recipients. Animals were bled at 8, 12, and 16 wk after transplantation for blood count and hematopoietic reconstitution by flow cytometry. (A and B) Two independent transplantations were performed; n = 20–22 mice/experiment. Data show mean ± SEM. (C) Cell cycle status, apoptosis, and necrosis in CLPs in OcnCre+/−;iDTR mutants and controls were assessed by flow cytometry. 2 independent experiments; n = 5–6 mice/group. Data show mean ± SEM.
