Ocn⁺ cell deletion decreases T cell competent lymphoid progenitors and T lineage cells. (A) Flow cytometric evaluation of lymphoid, myeloid, erythroid, and megakaryocytic mature cells and (B) T cell competent (Ly6D⁻) CLP, B potential enriched (Ly6D⁺) CLP, CMP, GMP, and MEP populations in the bone marrow of control and Ocn⁺ cell–deleted animals. Minimum of three independent experiments; n = 8–16 mice/group. Data show mean ± SEM. (C) Gene set enrichment analysis (GSEA) comparing Ly6D⁻ CLP versus multipotent progenitor (MPP) microarray data. Experiment was performed twice independently; n = 3/experiment. (D) Flow cytometric quantification of different stages of B progenitors and mature cells in the bone marrow of OcnCre;iDTR mutant and control littermates. Experiment was performed twice independently; n = 5–6 mice/group. Data show mean ± SEM. (E) Flow cytometric quantification of T cell subsets in the thymus. Minimum of four independent experiments; n = 8–12 mice/group. Data show mean ± SEM.