Figure 4.
Figure 4. Neutrophils accumulate at onset of disease in adjuvant-free models of EAE. (A–D) OSE mice were sacrificed when healthy (n = 5; white bars), within 2 d of the onset of clinical EAE (n = 5; black bars) or during the chronic stages of EAE (n = 4; gray bars). Peripheral blood cells (A), splenocytes (B), BM cells (C) and spinal cord–infiltrating cells (D) were collected. Neutrophils and monocytes were enumerated by flow cytometry. (E and F) WT mice were injected with IL-12–polarized (Th1; black bars) or IL-23–polarized (Th17; gray bars) MOG-specific T cells. At day 7 after transfer, blood (E) and spleens (F) were collected and neutrophils and monocytes were enumerated by flow cytometry. Data are pooled from four (Th17 transfers) or two (Th1 transfers) experiments (n ≥ 10 mice per group). All graphs indicate means; error bars denote SEM. *, P < 0.05; **, P < 0.005; ***, P = 0.001; ****, P < 0.0001, by two-way ANOVA, correcting for multiple comparisons.

Neutrophils accumulate at onset of disease in adjuvant-free models of EAE. (A–D) OSE mice were sacrificed when healthy (n = 5; white bars), within 2 d of the onset of clinical EAE (n = 5; black bars) or during the chronic stages of EAE (n = 4; gray bars). Peripheral blood cells (A), splenocytes (B), BM cells (C) and spinal cord–infiltrating cells (D) were collected. Neutrophils and monocytes were enumerated by flow cytometry. (E and F) WT mice were injected with IL-12–polarized (Th1; black bars) or IL-23–polarized (Th17; gray bars) MOG-specific T cells. At day 7 after transfer, blood (E) and spleens (F) were collected and neutrophils and monocytes were enumerated by flow cytometry. Data are pooled from four (Th17 transfers) or two (Th1 transfers) experiments (n ≥ 10 mice per group). All graphs indicate means; error bars denote SEM. *, P < 0.05; **, P < 0.005; ***, P = 0.001; ****, P < 0.0001, by two-way ANOVA, correcting for multiple comparisons.

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