Neutrophils and monocytes expand in the bone marrow after EAE induction. (A–C) WT mice were immunized with MOG_35-55 in CFA and injected with PTx on days 0 and 2 p.i. Bone marrow cells were flushed from femurs and tibiae of representative mice at serial time points and analyzed by flow cytometry to enumerate neutrophils (CD31⁻Ly6C^intLy6G⁺, black bars/closed circles), monocytes (CD31⁺Ly6C^hiLy6G⁻, gray bars and circles), and lymphocytes (CD31⁺Ly6C⁻, diagonal stripes/open triangles). (A) Percentage of leukocyte subsets within bone marrow cells. Data are representative of six experiments (n ≥ 3 per time point). (B and C) Absolute numbers of neutrophils (B) and monocytes and lymphocytes (C) recovered per mouse. Data are representative of nine experiments (n ≥ 3 per time point). All graphs indicate means; error bars denote SEM. *, P < 0.05; **, P < 0.01 compared with unimmunized mice.