Insulin–InsR signaling skews myeloid/lymphoid differentiation, but not myeloid-biased HSCs. (A and B) 10 CD150^highCD34⁻LSK or CD150^negCD34⁻LSK cells pretreated with 200 nM insulin for 18 h were transplanted together with 3 × 10⁵ CD45.1 BM cells into lethally irradiated mice, followed by examination of blood constitutions 16 wk later. Relative myeloid, B cell, and T cell compositions were presented. (C and D) 10 CD150^highCD34⁻LSK or CD150^negCD34⁻LSK cells from Insr^+/+ and Insr^−/− mice were transplanted together with 3 × 10⁵ CD45.1 BM cells into lethally irradiated mice, and assessed as in A. (E–H) 10 of the indicated cells were transplanted together with 3 × 10⁵ CD45.1 BM cells into lethally irradiated mice, followed by donor chimerism examination 16 wk later. (A–H) n = 15. (I) CD150^highCD34⁻LSKs were calculated in BM from Insr^+/+ and Insr^−/− mice. (J) CD34⁻Flk2⁻LSKs were further analyzed by surface marker CD150 and cells with distinct CD150 expression were calculated. (I and J) n = 6. Data are shown as means ± SD. Data are from experiments repeated three times with similar results.