Cell-autonomous defect in thymocyte maturation in Lrrc8a^−/− mice. (A–C) Representative FACS analysis of thymocytes with the percentage of cells found in each quadrant indicated (A), number of thymocyte subsets (B), and number of DN cell subsets (C) in the chimeras. (D and E) Percentage of annexin V⁺ cells (D) and of BrdU⁺ cells 3 h after i.p. injection of BrdU (E) in total thymocytes from the chimeras. (F) ³H-thymidine incorporation in thymocytes from the chimeras in response to medium (med), anti-CD3+IL-2, and PMA+ionomycin (P+I) stimulation after 72 h in culture. Data are representative of three independent experiments with one mouse per group (A–F). Mean and SEM are shown in B–F. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student’s t test). NS = not significant.