Figure 2. Trem2 expression in myeloid cells occurs with plaque association. (a) Trem2 expression is observed in myeloid cells (Iba1 positive) associated with plaques (stained with Methoxy-X04) in APP23 (shown) and APPPS1 mice (not depicted). Images are maximum projections of confocal z-stacks (insets show representative high-magnification images of a single confocal plane in plaque-associated cells). (b) Maximum projection of confocal z-stack in 4-mo-old APPPS1/TK− animals shows Iba1-positive cells expressing Trem2 in APPPS1/TK− animals, whereas nonplaque-associated infiltrating monocytes do not express Trem2 in APPPS1/TK+ mice. (c) APP23/TK− as well as APP23/TK+ animals repopulated before Aβ deposition show plaque-associated myeloid cells positive for Trem2 at the age of 10 mo. Bars: (a) 30 µm; (b and c) 20 µm. Immunostaining was performed for n ≥ 4 randomly chosen animals and replicated three times.
Figure 2.

Trem2 expression in myeloid cells occurs with plaque association. (a) Trem2 expression is observed in myeloid cells (Iba1 positive) associated with plaques (stained with Methoxy-X04) in APP23 (shown) and APPPS1 mice (not depicted). Images are maximum projections of confocal z-stacks (insets show representative high-magnification images of a single confocal plane in plaque-associated cells). (b) Maximum projection of confocal z-stack in 4-mo-old APPPS1/TK− animals shows Iba1-positive cells expressing Trem2 in APPPS1/TK− animals, whereas nonplaque-associated infiltrating monocytes do not express Trem2 in APPPS1/TK+ mice. (c) APP23/TK− as well as APP23/TK+ animals repopulated before Aβ deposition show plaque-associated myeloid cells positive for Trem2 at the age of 10 mo. Bars: (a) 30 µm; (b and c) 20 µm. Immunostaining was performed for n ≥ 4 randomly chosen animals and replicated three times.

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