Tamoxifen induced Cre activity in Fgd5^{ZsGreen•CreERT2/+} mice exclusively targets CD150⁺ HSCs. (A) Flow plots of tamoxifen-treated Fgd5^{ZsGreen•CreERT2/+}:Rosa26^+/+ and Fgd5^{ZsGreen•CreERT2/+}:Rosa26^{LoxSTOPLoxdTomato/+} mice showing primitive LSK compartment subfractionated into CD150- and CD150⁺ population (left), with each population then gated through ZsGreen and dTomato (right). (B) Cre-mediated dTomato expression in control Fgd5^{ZsGreen•CreERT2/+}; Rosa26^+/+ and experimental Fgd5^{ZsGreen•CreERT2/+}; Rosa26^{loxSTOPloxdTomato/+} mice upon tamoxifen induction shown in the indicated BM populations including Lineage⁻, Lineage⁺, Lin⁻cKit⁺Sca1⁻ (myeloid progenitors), LSKCD150⁻ cells, LSK, and LSKCD150⁺ HSC (n = 3 per group).