All HSCs are labeled by Fgd5^mCherry. (A) Gating strategy used for sorting reporter-positive and -negative fractions set using Fgd5^+/+ (top) and Fgd5^mCherry/+ (bottom) mice. (B and C) Transplantation of 100 mCherry⁺ (red) or 100,000 mCherry⁻ (gray) cells from Fgd5^mCherry/+ mice showing total donor reconstitution over the time course of transplantation in experiment 1 (B) and experiment 2 (C). (D) Peripheral blood analysis of representative recipients from experiment 2 showing donor reconstitution (CD45.2) and contribution to granulocytes (Mac1⁺Gr1⁺), macrophages/monocytes (Mac1⁺Gr1⁻), B cells (Mac1⁻, B220⁺CD3⁻) and T cells (Mac1⁺, B220-CD3⁺) analyzed 24-wk after transplant. (E and F) Peripheral blood analysis showing granulocyte chimerism plotted against the time course of transplantation in experiments 1 (E) and 2 (F). n = 5 per group in experiment 1; n = 4 (Fgd5^mCherry/+); n = 5 (Fgd5^+/+) in experiment 2.