Labeling of HSCs by Fgd5^mCherry/+ is retained after transplantation. (A and B) BM analysis of recipient mice transplanted with 120 LSKCD48⁻CD150⁺ HSCs (HSC^Slam) from wild-type mice (A) or 120 mCherry⁺ cells from Fgd5^mCherry mice (B), analyzed 8 mo after transplant showing donor-derived (CD45.2) chimerism and contribution to BM compartments revealed by co-staining with antibodies against lineage, c-Kit, Sca1, flk2, and CD34. (C) Histograms showing mCherry signal in the indicated cell fractions from the BM of recipient mice transplanted with 120 HSC^Slam cells from wild-type mice (left) or 120 mCherry⁺ cells from Fgd5^mCherry mice 8 mo after transplant. Representative data from n = 4 mice per group.