Reporter-labeled cells in BM of Fgd5^mCherry/+ mice are synonymous with immunophenotypic HSCs. (A) Histograms (colored) of reporter expression within immunophenotypic HSCs (LSKCD48⁻CD150⁺) from the BM of mice targeted at Clec1a^eGFP, Sult1a1^eGFP, and Fgd5^mCherry in comparison to the wild-type background (gray). (B) BM mCherry⁺ (red histograms) and mCherry⁻ (black histograms) cells of Fgd5^mCherry/+ mice co-stained and gated individually (top) or sequentially (bottom) through lineage (Ter119, Mac-1, Gr-1, B220, CD3, CD4, and CD8), c-Kit, Sca1, CD48, and CD150. (C–F) Sub-fractionation of primitive LSK cells from Fgd5^mCherry/+ (red histograms) or Fgd5^+/+ (gray histograms) mice into immunophenotypic HSCs (right, green gates) and multipotent progenitors (blue and black gates) by (C) CD150 and CD48, (D) CD34 and flk2, (E) PROCR and CD34, and (F) ESAM and CD34. Percentage mCherry⁺ ± SD is shown and a minimum of n = 3 mice for each stain were analyzed.