Identification of HSC-specific genes. (A) Schematic representation of the hematopoietic hierarchy showing cell types with expression profiles used in this study framed in red. (B) Heat map showing relative expression (from red, high, to blue, low) of genes identified as cell type specific in the indicated cell types, with asterisks (*) marking genes with well-established roles in the given cell type. (C) Heat map showing relative expression of representative genes identified as HSC-specific (for complete list see Fig. S1). (D) Venn diagram of HSC-enriched gene sets published in Gazit et al. (2013) (2013), Chambers et al. (2007a), and this study. Genes expressed >2.3-fold higher in HSCs relative to downstream populations examined in each study were selected from the current study and also the Chambers et al. (2007a) dataset. Percentage overlap of our HSC-enriched gene set with others is presented on the bottom.