BRAFV600E^CD11c mice develop an aggravated phenotype with massive infiltration of MHCII⁺ CD11c⁺ langerin-expressing cells in peripheral tissues. (A) Representative pictures of the spleen and liver of BRAFV600E^CD11c mice (right) and control littermates (left) at 12 wk of age (n = 16 per group). (B) Representative H&E staining of liver and lung tissue sections of BRAFV600E^CD11c mice and corresponding controls at the indicated ages (arrow marks multinucleated giant cells; bars, 100 µm; n = 5–11 animals per group). (C) H&E staining of back skin tissue sections of 12-wk-old BRAFV600E^CD11c mice and controls (arrows indicate multinucleated giant cells; bars, 100 µm; n = 4 per group). (D) Absolute numbers and percentages of MHC II⁺ CD11c⁺ DC among total CD45⁺ lung and liver cells in 12-wk-old BRAFV600E^CD11c mice and control littermates were determined by multicolor flow cytometry (all cells pregated on singlets, viable cells, n = 3–5 per group, representative of three independent experiments). (E) Representative image of frozen liver tissue section isolated from the liver of 16-wk-old BRAFV600E^CD11c mice stained with anti-langerin mAb (left) and anti-CD11c mAb (right) or with both langerin and CD11c mAb (merged) and analyzed by confocal microscopy (bars, 100 µm). (F) Langerin mRNA expression in whole liver tissue of BRAFV600E^CD11c mice and corresponding controls was analyzed by qPCR (normalized to Gapdh mRNA expression, n = 6–11 per group). All data are shown as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.