BRAFV600E^langerin mice spontaneously developed progressive accumulation of inflammatory infiltrates in peripheral tissue. (A and B) BRAFV600E^langerin mice were generated as described in Materials and methods and sacrificed at 12 wk (A) or 20 wk (B). Representative lung and liver sections were stained by H&E (in total at least 4–5 mice per group were analyzed; arrows indicate inflammatory infiltrates; bars, 100 µm). (C and D) Lung and liver tissue of BRAFV600E^langerin mice and control littermates were harvested at 12 wk of age and single cells suspensions were analyzed by multicolor flow cytometry analysis after digestion with collagenase. Absolute numbers of total MHCII⁺ CD11c⁺ DCs, MHCII⁺ CD11c⁺ CD103⁺ DCs, and MHCII⁺ CD11c⁺ CD11b⁺ DCs are presented (C, all cells are pregated on singlets, viable CD45⁺ cells, n = 3 per group, representative of two independent experiments). Absolute number of tissue infiltrating total hematopoietic CD45⁺ cells, F4/80⁺ macrophages, total CD3⁺ NK1.1⁻ T cells, total CD3⁺ NK1.1⁻ CD4⁺ CD8⁻ T cells, total CD3⁺ NK1.1⁻ CD4⁺ Foxp3⁺ T reg cells, total CD19⁺ B220⁺ B cells, and total CD3⁻ NK1.1⁺ NK cells are depicted in D (all cells are pregated on singlets, viable CD45⁺ cells, n = 3 per group, representative of two independent experiments). (E) Representative picture of IF staining for langerin in liver lesion of 12-wk-old BRAFV600E^langerin mouse. Bars, 100 µm. Data are shown as mean ± SEM. *, P < 0.05.