Figure 4. BRAF-V600E localizes to CD34... | Rockefeller University Press

Figure 4.

$Figure 4. BRAF-V600E localizes to CD34+ hematopoietic progenitor cells in BM. (A) Sorting strategy for BM aspirate cells into HSPCs (CD34+) and monocytes (CD14+). (B) CD34+ and CD14+ cells were sorted from BM aspirate fractions, gDNA was purified and amplified, and then the BRAF-V600E allele was detected by qPCR. (C) Percentage of CD34+ cells with BRAF-V600E in BM aspirate and the percentage of BRAF-V600E cells obtained from CD34+ HSPC-derived colonies generated in CFU Assay in two LCH patients. (D) PBMCs were sorted by FACS into CD3+ and CD19+ fractions. Percentage of peripheral B and T cells with BRAF-V600E in different LCH patients. (B–D: every bar represents a single sorted blood sample; technical duplicates were used in all experiments.)$

BRAF-V600E localizes to CD34⁺ hematopoietic progenitor cells in BM. (A) Sorting strategy for BM aspirate cells into HSPCs (CD34⁺) and monocytes (CD14⁺). (B) CD34⁺ and CD14⁺ cells were sorted from BM aspirate fractions, gDNA was purified and amplified, and then the BRAF-V600E allele was detected by qPCR. (C) Percentage of CD34⁺ cells with BRAF-V600E in BM aspirate and the percentage of BRAF-V600E cells obtained from CD34⁺ HSPC-derived colonies generated in CFU Assay in two LCH patients. (D) PBMCs were sorted by FACS into CD3⁺ and CD19⁺ fractions. Percentage of peripheral B and T cells with BRAF-V600E in different LCH patients. (B–D: every bar represents a single sorted blood sample; technical duplicates were used in all experiments.)